Production of tyrosine hydroxylase positive neurons

摘要

A method for producing neurons Express tyrosine hydroxylase (TH) by neural stem cells to the action of fgf-1, an Activator of protein kinase a, an Activator of protein kinase C / L - dopa and dopamine.When forskolin was used as an Activator of protein kinase are necessary, only low levels of fgf-1 and forskolin to efficiently produce TH positive neurons from fetal or adult neural stem cells.Also provided are compositions which are used to produce TH positive neurons and the resulting neuronal cell culture, as well as a method for treating diseases or conditions associated with neuronal loss or dysfunction of dopamine.