A fusion protein to release a compound to a cell, a method for releasing the compound, a method for identifying a Membrane penetrating Peptide, and the use of a fusion protein for the manufacture of an agent

摘要

It is aimed at the peptide entering the membrane, as an effective intracellular delivery device for compounds of interest in vivo, previvo and in vitro. It is also aimed at fusion proteins, so as to release a compound of interest to cells, including membrane penetrating proteins and compounds of interest. In one aspect, the membrane penetrating peptide is derived from the nuclear localization sequence, overlaps with the nuclear localization sequence of mammalian protein or yeast, or contains the n (x-x-x-x) sequence, where n is an integer from 1 to 7,X is always independent of the group to which arginina, histidina or lysine belongs On the other hand, the nuclear location sequence is produced by transcripts of nuclear protein or human protein. On the other hand, mammalian periodic proteins are human periodic proteins. In one aspect, related compounds are associated with membrane penetrating peptides, either directly or through binders, such as amino acids or peptides. per contraThe object of the invention is to release a compound associated with the fusion peptide of the membrane penetrator. On the other hand, fusion protein is used to make a preparation that can release a compound of interest to a pre living cell, implant the cell into the patient's body, and make a preparation that can release the compound of interest to the living cell. Finally, a method is used to identify membrane penetrating peptides with detectable proteins,Heterozygous binding peptides were added to the cell, and whether the binding peptides were placed in the cytochrome and / or cell core was determined.