Method for the Treatment or prophylaxis of a viral infection by Flaviviridae, Orthomyxoviridae, Paramyxoviridae, or abnormal Cell Proliferation and use of modified Nucleosides

摘要

Method for treating a Flaviviridae infection by a virus (including BVDV and HCV) (including influenza Orthomyxoviridae, paramyxoviridae (A and b) or including RSV), or conditions associated with an abnormal Cell Proliferation, in a host, including Animals, and especially Human Beings, which utilizes a nucleoside of formula (1S, 1b, 1c) - (2S, 2b, 2c)Or its enantiomer BETA L or its acceptable Salt or prodrug for pharmaceutical use: D, where each is Hydrogen, alkyl, acyl, monophosphate, diphosphate and triphosphate guanosine diphosphate Ester, Ester, Ester or amino acid phospholipid triphosphate; each W1 and W2 is CH or independently N; each x1 and x2 is independently Hydrogen, halogen (F, CL, Br or i), NH2, nhr4, nr4r4 ', nhor4,Nr4nr4 'R4', 'Oh, or4, SH or SR4, Y1 is o, s or Z; each is CH2 or NH; each R1 and R1 is independently Hydrogen, Lower alkyl, aryl alquinilo alquenilo Lower, Lower, alkylaryl, halogen (F, CL, Br or i), NH2, NHR5, nr5r5', nhor5, nr5nhr5 R5, nr5nr5 ',' 'Oh, OR5, sh, SR5, NO2, n Or, CH2OH, ch2or5, CO2H, co2r5, CONH2, conhr5,Conr5r5 'cn' Or 'independently; each R2 and R2 is Hydrogen or Halogen (F, CL, Br or i), Oh, sh, OCH3, Sch3, NH2, nhch3 = CH2, CH, CN, ch2nh2, CH2OH CO2H; R3, R3' and each is independently Hydrogen or Halogen (F, CL BR, or i), Oh, sh, Sch3, NH2 OCH3, CH3, C2H5, nhch3, = CH2, CH, CN, ch2nh2, CH2O H, CO2H; each R4, R4, R4, R5, R6, '' 'and' 'regardless R5 is Hydrogen, Lower alkyl,Lower alquenilo, aryl or arylalkyl as insustituido or substituted phenyl or benzyl; so that the General formula for the nucleoside (1S, 1b, 1c) or (2S, 2b, 2c) at least one of R2 and R2 is Hydrogen and at least one of R3 and R3 is Hydrogen, also it provides an effective process for Quantification of viral load and, in particular, The Viral Load of BVDV, HCV and West Nile virus.In a host, using a quantitative polymerase chain reaction (Real Time rt-pcr '' ''). In addition, we describe fluorescence probe molecules that can produce in proportion to the amount of virus in a sample.