Imunoenzimu00e1tica technique (ELISA) in reverse for the quantification of specific allergens IgE

摘要

"Imunoenzimu00e1tica technique (ELISA) in reverse for the quantification of IgE specific allergens.The present invention, ELISA, reverse.Aims to quantify levels of serum specific IgE antibodies to the allergens as a potential tool for the etiological diagnosis of hipersensiblidade in patients with the disease alu00e9rgicAs for the monitoring of allergen immunotherapy in these patients.In this way, anti allergens specific monoclonal antibodies, such as anti Der p 2.They are linked to microtitre ELISA plates to capture the relevant allergen (Der p 2) present in the crude extract of Dermatophagoides pteronyssinus,Subsequently interacts with specific IgE antibodies in samples of sera from allergic patients.These antibodies are then detected by addition of anti human IgE antibody policional biotinilado and subsequently estreptavidina peroxidase conjugate.The revelation of the reaction is performed by adding the enzyme substrate (ABTS) and the absorbance determined reader microplates at 405 nm.When compared with the conventional ELISA (CELISA) that uses the allergen Der p 2 (native or recombinant purified) directly on the awareness of microplates.Reverse ELISA proved to be more sensitive CELISA both by the percentage of positive sera (70.8% versus 52.1%) and the levels of specific IgE to Der p 2 (28.4 versus 4.5 L / ml) in asthma patients with positive skin test to d. pteronyssinus.In conclusion, reverse ELISA proved to be a sensitive method to quantify serum IgE and alternative specific allergens.Contributing to the etiological diagnosis of hypersensitivity in patients with respiratory allergic disease and provide valuable information for the monitoring of immunotherapy with peptu00eddeThe relevant or allergens.