Monoclonal antibody eletronegativa anti LDL (LDL -); the process of obtaining antibody; sets for detection of LDL in plasma diagnostic blood and in atherosclerotic lesions; composition farmacu00eauPractice for combat and / or prevention to cru00c8nico degenerative diseases and method of application of the drugs prepared at the base of the antibody in the control and prevention of diseases cru00c8nico degenerative

摘要

"Eletronegativa monoclonal antibody anti LDL (LDL); the process of obtaining antibody; sets for detection of LDL in plasma diagnosis sanguu00ecneo and in atherosclerotic lesions; composition farmacu00eauPractice for combat and / or prevention to cru00c8nico degenerative diseases and method of application of the drugs prepared at the base of the antibody in the control and prevention of diseases cru00c8nico degenerative.It is the invention of antibody Monocional eletronegativa anti LDL (LDL -) produced in vivo and its production process.Achieved through the following steps: 1 isolation of LDL ^ - ^; 2 - immunization of BALB / c mice; 3 - check the answer policional anti LDL ^ - ^; 4 - cell fusion; 5 - screening of clones; 6 - CLonagem and sub cloning; 7 - the production of ascites; 8 - purification of monoclonal antibody anti LDL ^ - ^; 9 - isotipagem; 10 - Determination of cross reactivity.From the Monocional antibody obtained, have been standardized sets of diagnosis for detection of LDL ^ - ^ in plasma sangu00b3u00edneo and in atherosclerotic lesions.In addition to the pharmaceutical composition and method of application of the drugs prepared from this antibody, for the treatment and prevention of chronic degenerative diseases, such as atherosclerosis.The invention is used in the areas of Biochemistry and pharmacy and human and veterinary medicine.For diagnosis of atherosclerosis and therapeutic agent against chronic degenerative diseases and treatment.