Process for purification of human prolactin using resin affinity chromatography on immobilized metal

摘要

Process for purification of human prolactin using resin affinity chromatography on immobilized metal ".The invention relates to a process for the purification of human prolactin using resin affinity chromatography on immobilized metal.This process was explored the binding capacity of the hPRL divalent metals.This feature gives the process a greater specificity of binding of hPRL to a resin affinity chromatography on immobilized metal (IMAC), which was used as the metal.The Ni ^ 2 + ^. By the use of the process according to the invention, the values of both yield and purity of the hPRL recombinant bacterial origin obtained were exceptionally high.The invention also relates to a second chromatographic step for purification of hPRL resin molecular exclusion, which obtains a product almost pure.Perfectly usable for diagnostic or research purposes.