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Process for purification of human prolactin using resin affinity chromatography on immobilized metal
Process for purification of human prolactin using resin affinity chromatography on immobilized metal
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机译:在固定的金属上使用树脂亲和层析纯化人催乳素的方法
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摘要
Process for purification of human prolactin using resin affinity chromatography on immobilized metal ".The invention relates to a process for the purification of human prolactin using resin affinity chromatography on immobilized metal.This process was explored the binding capacity of the hPRL divalent metals.This feature gives the process a greater specificity of binding of hPRL to a resin affinity chromatography on immobilized metal (IMAC), which was used as the metal.The Ni ^ 2 + ^. By the use of the process according to the invention, the values of both yield and purity of the hPRL recombinant bacterial origin obtained were exceptionally high.The invention also relates to a second chromatographic step for purification of hPRL resin molecular exclusion, which obtains a product almost pure.Perfectly usable for diagnostic or research purposes.
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