a method for determining the degree of u043cu0435u0442u0438u043bu0438u0440u043eu0432u0430u043du0438u044f specific u0446u0438u0442u043eu0437u0438u043du043eu0432 in genome dna in the context of the sequence 5 '- 3' CPG

摘要

the application describes the method of determining the u043cu0435u0442u0438u043bu0438u0440u043eu0432u0430u043du0438u044f specific u0446u0438u0442u043eu0437u0438u043du0430 in the context of the sequence 5 '- 3' CpG sample genome dna.at the first stage of genome dna subjected to chemical treatment so as to cause u0446u0438u0442u043eu0437u0438u043du0430 except for 5 - u043cu0435u0442u0438u043bu0446u0438u0442u043eu0437u0438u043du0430, converted to uracil.further, the segments of the genome dna, which contain the specific cytosine, u0430u043cu043fu043bu0438u0444u0438u0446u0438u0440u0443u044eu0442,supply u0430u043cu043fu043bu0438u0444u0438u0446u0438u0440u043eu0432u0430u043du043du044bu0435 products label detection, and at the following stages of the degree of hybridization u0430u043cu043fu043bu0438u0444u0438u0446u0438u0440u043eu0432u0430u043du043du044bu0445 products define two classes u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u043eu0432 u0430u043cu043fu043bu0438u0444 identification tagsu0438u0446u0438u0440u043eu0432u0430u043du043du044bu0445 products.conclusion the extent of u043cu0435u0442u0438u043bu0438u0440u043eu0432u0430u043du0438u044f the specific u0446u0438u0442u043eu0437u0438u043du0430 sample genome dna is made of the ratio of tags identified in the two classes of u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438 dov, as a consequence of hybridization.the international search report was published 2004.02.05.