Minimal promoters comprising a promoter not linked to its native enhancer and such minimal promoters linked to an antigen

摘要

Minimal promoter sequences are described. Reagents including a nucleic acid molecule which contains these minimal promoter sequences are also described. Methods for constructing these reagents, and methods for using these reagents are also described. Particularly described are minimal promoters, consisting of a promoter sequence not coupled to its native enhancer sequence, operably linked to a coding sequence for an antigen which are useful as a vaccine for immunizing a mammalian subject.