Described is a method for producing mature somatic embryos with cryopreserved genotypes and applied successfully to a wide range of genotypes and families. The method comprises the establishment and continuous proliferation of at least 40% of induced genotypes, treatment for reducing the proliferation rate, treatment for initiating the development of the immature somatic embryos, maturing the somatic embryos of at least 80% of treated genotypes corresponding to more than 90% of families analysed. This is achieved mainly by simultaneous application of different ammonium/nitrate ratios during the steps included in the method. Fig. 1a represents the selection of media in order to establish and get continuing proliferation. In which is demonstrated each genotype has a different proliferation capacity depending on ammonium to nitrate molar ratio (A').
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