A kit that detects directly the pathogenicity of the pathogenic influenza A subtype H5 virus

摘要

Current methods for detecting influenza A subtype H5 virus, for example cell culture, haemagglutination- inhibition, fluorescent antibody and enzyme immunoassay, and reverse transcriptase polymerase chain reaction (RT- PCR) may have the disadvantages of low sensitivity and low specificity. Furthermore, such methods are relatively difficult to use, and may not be suitable for routine detection on a daily basis. A kit for detecting H5 virus is described. The kit provides a user-friendly alternative that is relatively more sensitive and specific to H5 virus. The kit utilizes two specifically designed primers A and B for the replication of H5 virus, and a specific capture probe for immobilizing the amplified viral RNA. An additional primer C is also designed for the detection of pathogenic H5 virus. The detection of H5 virus by the detection kit may be accomplished within one day.