PROCESS FOR THE PRODUCTION OF LIPSTATIN AND TETRAHYDROLIPSTATIN

摘要

A PROCESS FOR THE FERMENTATIVE PRODUCTION OF LIPSTATIN, WHICH PROCESS COMPRISES A) AEROBICALLY CULTIVATING A MICRO-ORGANISM OF THE ORDER OF ACTINOMYCETES WHICH PRODUCES LIPSTATIN, IN AN AQUEOUS CULTURE MEDIUM WHICH IS SUBSTANTIALLY FREE OF FATS AND OILS, AND WHICH CONTAINS SUITABLE CARBON AND NITROGEN SOURCES AND INORGANIC SALTS, UNTIL THE INITIAL GROWTH PHASE IS SUBSTANTIALLY FINISHED AND SUFFICIENT CELL MASS HAS BEEN PRODUCED, AND B) ADDING TO THE BROTH LINOLEIC ACID, PREFERABLY TOGETHER WITH CAPRYLIC ACID, [WHEREIN PART OR THE TOTALITY OF THE LINOLEIC ACID AND/OR OF THE CAPRYLIC ACID CAN BE REPLACED BY THE CORRESPONDING ESTER(S) AND/OR SALT(S)], AND NFORMYL- L-LEUCINE OR PREFERABLY L-LEUCINE, THE LINOLEIC ACID OR ITS ESTER(S) OR SALT(S) BEING STABILIZED BY AN ANTIOXIDANT. LIPSTATIN CAN BE ISOLATED FROM THE BROTH AND HYDROGENATED TO TETRAHYDROLIPSTATIN.