Conjugates qumioluminiscente energy transfer and applications as labels in binding assays.

摘要

The invention relates to a new class of chemiluminescent compounds or benzacridinium acridinium through forming a conjugate intramolecular energy transfer (ETC) between the acridinium or benzacridinium compounds and a luminophore. The invention also relates to a method for extending the wavelength of emission esters acridinium or benzacridinium to further reduce or eliminate the emission spectral overlap between the esters of acridinium (DMAE) and esters benzacridinium (LEAE) polysubstituted parents. ETCs retain the unique compounds acridinium and benzacridinium including complete light emission in a very short time, spectrum monophasic emission simplicity starting mechanism, ability to mark the biological molecules of interest associated properties to form a tracer and good stability. Moreover, the range of the emission spectrum of a compound or benzacridinium acridinium can now deflect at will and with a major leap through the choice of a luminophore as an integral part of an ETC molecule The invention relates to conjugates chemiluminescent labeled including means acridinium or benzacridinium covalently attached to a luminophore via a spacer, said element is further conjugated to a molecule of interest, wherein said spacer is of an appropriate length for allowing said excited species generated in this way sufficient energy to transfer said luminophore, resulting in the emission of light in the spectral region of said luminophore. The invention also relates to binding assays using the above conjugates, kits including the aforementioned test conjugates and methods for preparing conjugates.