Substantially pure zonulin, a physiological modulator of mammalian tight junctions.

摘要

Zonulin having an apparent molecular weight about 47 kDa as determined by electrophoresis in SDS-polyacrylamide gel which is recognized by both polyclonal anti-tau as polyclonal anti-ZOT, and is capable of reversibly opening mammalian tight junctions, and wherein said zonulin has an amino acid sequence N-terminal selected from the group consisting of: (1) Asn Gln Arg Pro Pro Pro Ala Gly Val Thr Ala Tyr Asp Tyr Leu Val Ile Gln (SEQ ID NO: 1); (2) Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu (SEQ ID NO: 2) (3) Val Thr Phe Tyr Thr Asp Ala Val Ser (SEQ ID NO: 3); (4) Met Leu Gln Lys Ala Glu Gly Ser Gly Val Leu Val Gln Pro Gly Xaa Ser Asn Arg Leu (SEQ ID NO: 4); and (5) Glu Val Gln Leu Val Glu Ser Gly Gly Xaa Leu (SEQ ID NO: 7)