Trap systems interactions to detect protein interactions.

摘要

Herein disclosed is a method of determining whether a first protein is capable SE INTERACT PHYSICALLY with a second protein, comprising: (a) providing a host cell containing (i) A reporter gene operably linked SITE-binding form PROTEIN ; (II) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently attached to a connecting structure that is capable of specifically binding to binding site of the protein; And (iii) a second fusion gene expressing a second fusion protein, THE SECOND INCLUDING THE FUSION PROTEIN second protein covalently bound to a structure of gene activation and being constrained accordingly; AND (B) MEDIAR reporter gene expression as a measure of an interaction between first and second proteins. ALSO DESCRIBED TEST METHODS PROTEIN INTERACTIONS, and identifying antagonists and agonists PROTEIN INTERACTIONS. Also discussed proteins isolated by these methods. FINALLY, STOCKS DESCRIBED eukaryotic cells, each cell HAVING A DNA molecule encoding a peptide RECOMBINANT INTRACELLULAR constrained accordingly.