GLIA ACTIVATING FACTOR AND ITS PRODUCTION

摘要

Disclosed are (1) a novel glia activating factor whichhas glial cell growth promoting activity; (2) a gliaactivating factor containing a polypeptide represented by thefollowing amino acid sequence:Leu Asp His Leu Lys Gly Ile Leu Arg Arg ArgGln Leu Tyr Cys Arg Thr Gly Phe His Leu Glu Ile Phe Pro AsnGly Thr Ile Gln Gly Thr Arg Lys Asp His Ser Arg Phe Gly IleLeu Glu Phe Ile Ser Ile Ala Val Gly Leu Val Ser Ile Arg GlyVal Asp Ser Gly Leu Tyr Leu Gly Met Asn Glu Lys Gly Glu LeuTyr Gly Ser Glu Lys Leu Thr Gln Glu Cys Val Phe Arg Glu GlnPhe Glu Glu Asn Trp Tyr Asn Thr Tyr Ser Ser Asn Leu Tyr LysHis Val Asp Thr Gly Arg Arg Tyr Tyr Val Ala Leu Asn Lys AspGly Thr Pro Arg Glu Gly Thr Arg Thr Lys Arg His Gln Lys PheThr His Phe Leu Pro Arg Pro VaZ Asp Pro Asp ;or a mutein thereof having activity of the factor:(3) a DNA containing a following polynucleotide coding for aglia activating factor:TTG GATCATTTAA AGGGGATTCTCAGGCGGAGG CAGCTATACT GCAGGACTGG ATTTCACTTA GAAATCTTCCCCAATGGTAC TATCCAGGGA ACCAGGAAAG ACCACAGCCG ATTTGGCATTCTGGAATTTA TCAGTATAGC AGTGGGCCTG GTCAGCATTC GAGGCGTGGACAGTGGACTC TACCTCGGGA TGAATGAGAA GGGGGAGCTG TATGGATCAGAAAAACTAAC CCAAGAGTGT GTATTCAGAG AACAGTTCGA AGAAAACTGGTATAATACGT ACTCGTCAAA CCTATATAAG CACGTGGACA CTGGAAGGCGATACTATGTT GCATTAAATA AAGATGGGAC CCCGAGAGAA GGGACTAGGACTAAACGGCA CCAGAAATTC ACACATTTTT TACCTAGACC AGTGGACCCC GAC.(4) a host transformed with a vector containing the DNAdescribed in (3); and (5) a method for producing the aboveglia activating factor which comprises cultivating thetransformant described in (4) in a medium, accumulating theglia activating factor in a culture broth, and collecting theresulting glia activating factor.