ADENO-ASSOCIATED VIRUS VECTOR INHIBITING GENE'S FUNCTION BY RNAI

摘要

The plasmid carrying RNAi nucleotide fragment is transfected in the in-vitro cultured cell or animal body for continuously copying RNAi nucleotide fragments. Said fragment is encapsuled to become a recombinant gland-associated virus carrier carrying RNAi nucleotide fragment for gene transfer and copying. A short hair pin loop expression plasmid for suppressing the expression of leuciferinase in BHK-21 cell is configured. Said plasmid pSNAV/U6/Luc has high suppressing effect on leuciferinase (50-70%).