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Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
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机译:通过使用可调节的稳态报道基因高效生成稳定表达细胞系
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摘要
The present invention provides methods for site-specific recombination in a cell, as well as vectors which can be employed in such methods. The methods and vectors of the present invention can be used to obtain persistent gene expression in a cell and to modulate gene expression. One preferred method according to the invention comprises contacting a cell with a vector comprising an origin of replication functional in mammalian cells located between first and second recombining sites located in parallel. Another preferred method comprises, in part, contacting a cell with a vector comprising first and second recombining sites in antiparallel orientations such that the vector is internalized by the cell. In both methods, the cell is further provided with a site-specific recombinase that effects recombination between the first and second recombining sites of the vector.
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