SCREENING METHODS BASED ON CITED FAMILY PROTEINS

摘要

There is provided an in vitro method for identifying compounds which block the interaction between a CITED family protein and a TFAP2 protein and therefore have potential pharmacological activity in the treatment of cancer, which method comprises: forming a mixture comprising a first protein component comprising a CITED protein, a second protein component comprising a TFAP2 protein, and a candidate compound, incubating the mixture under conditions which, in the absence of the candidate compound, would permit binding of the CITED protein to the TFAP2 protein, and detecting any binding of the CITED protein to the TFAP2 protein; wherein compounds which block binding of the CITED protein to the TFAP2 protein are identified as having potential pharmacological activity in the treatment of breast cancer.