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Immobilised G-protein coupled receptors on an artificial lipid membrane of a liquid chromatography stationary phase and use thereof for screening
Immobilised G-protein coupled receptors on an artificial lipid membrane of a liquid chromatography stationary phase and use thereof for screening
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机译:固定在液相色谱固定相人工脂质膜上的G蛋白偶联受体及其在筛选中的用途
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摘要
This invention provides an immobilized G-protein coupled receptor (GPCR) on a support in a liquid chromatography system. The method of the invention provides means of evaluating the attachment of an agent to a GPCR comprising the steps of: (a) immobilizing a GPCR on an artificial membrane support in a column, (b) exposing the support with the GPCR to test the agent at vaiying concentrations in a liquid chromatography system, (c) eluting the test agent from the column, and (d) evaluating the elution profile of the test material from the column. Using this method, it is possible to evaluate the interaction of the test agent with the GPCR. Following elution. it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.
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