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METHODS USING FLUORESCENCE ENERGY TRANSFER PROBES FOR DETECTING CLEAVAGE OF NUCLEIC ACIDS
METHODS USING FLUORESCENCE ENERGY TRANSFER PROBES FOR DETECTING CLEAVAGE OF NUCLEIC ACIDS
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机译:荧光能量转移探针检测核酸裂解的方法
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摘要
Modified hairpin-forming oligonucleotide to continuously assess nucleotide cleavage by enediynes and other nucleic acid cleavage agents are provided. These oligonucleotide probes, which are also referred to herein as "molecular break lights," are also useful for continuous assessment of protection of nucleotides from cleavage agents. Probes according to the present invention are useful in assays; improved assays, including multiplexed assays, utilizing such pairs of molecules or moieties; and assay kits that include such pairs. Methods of using the probes are also provided.
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