GENE EXPRESSION VECTOR USING ECHINUS-ORIGIN INSULATORS

摘要

It is attempted to develop a vector which shows stable expression while avoiding the inactivation of a transferred gene by using echinus-origin ARS gene insulators. Namely, vectors expressing a marker gene are constructed and transferred into cultured cells. Then functions of vectors having insulators ligated thereto are analyzed. As a result, it is revealed that, by ligating two insulators in a specific direction to an expression cassette, a gene can be stably expressed and the expression dose can be elevated 130-fold or more compared with the case with the ligation of no insulator. Thus, the efficaciousness of the thus constructed vector is clarified.