NESTED METHYLATION-SPECIFIC POLYMERASE CHAIN REACTION CANCER DETECTION METHOD

摘要

Molecule is based in the method for label, for monitoring and detecting the people of cancer. The abnormal methylation of gene promoter is the label cancer risk mankind. Two-stage, ' ' nesting polymerase chain reaction method, it discloses one kind and is used to detect methylated DNA sequence in sufficiently high level of sensitivity, to allow in screening for cancer biologicfluid sample, such as saliva, what is obtained is non-invasive. This method is for detecting abnormal methylation p16 genes, 6-methylguanine-DNA methyl transferase genes of O, Death-associated protein kinase gene, 1 gene of RAS-associated families or other gene promoters. The selective mechanisms lung and other cancers that this method provides potentially strong approach based on population.