ANALYSIS OF THE PROTEOME OF A LIVING CELL BY LABELLING THE PROTEINS IN THE INTACT CELL

摘要

This invention provides a method for the analysis of a protein population of a living cell. It comprises the steps of a) contacting intact cells with a labeling compound comprising a protein-specific irreversible binding element and a reactive functionality Y and subsequently, b) adding a ligation-purification device comprising a purification handle and a reaction partner for said reactive functionality Y, c) purifying by means of said purification handle, and d) analyzing the material obtained from step c. Advantageously in this method the proteins of interest interact with the labeling compound in their actual working environment in an intact living cell. Labeling proteins by contacting intact living cells with a labeling compound, in vivo labeling, is more effective indicating improved conditions and circumstances for binding to proteins of interest in living cells as compared to the in vitro labeling.