DEVICE AND METHOD FOR THE OPTIMISED PREPARATION OF NUTRIENTS AND OXYGEN FOR MICROBIAL SURFACE CULTURES

摘要

The invention relates to a device and a method for the optimised preparation of nutrients for microbial surface cultures. The aim of the invention, taking careful consideration of the growth limitation of the cultivated micro-organisms, resulting e.g. from the formation of solid products (e.g. cellulose), is to overcome said limitation by means of optimised preparation of nutrients, in such a way that an optimum supply of nutrients and oxygen from micro-organisms of emerged cultures leads to the maximisation of the product yield, in order to achieve, for example, according to the type of culture, the production of homogeneous layers of cellulose having an unlimited thickness, or a high concentration of dissolved target products. To this end, a device is provided which consists of a culture vessel (1) surrounding a nutrient solution chamber (11) and an air chamber (12), a substrate aerosol producer (2), a substrate aerosol distributor (3) and a substrate aerosol evacuating device (4), said substrate aerosol producer (2), substrate aerosol distributor (3) and substrate aerosol evacuating device (4) being arranged in the air chamber (12) which is connected to a nutrient solution chamber (11) by means of a boundary surface (a). Said boundary surface (a) can be arranged between the bottom region and the covering region of the culture vessel (1) and the substrate aerosol producer (2) is arranged on the side of the substrate aerosol distributor (3) facing the boundary surface (a), said distributor being situated between the boundary surface (a) and the covering region of the culture vessel (1). The inventive device is used to pass the nutrient solution from the nutrient solution supply vessel (6) and air through the sterile filter (5), then through the substrate aerosol producer (2), via the nozzle (31), and through the slit distributor (32), said mixture being sprayed past the same into the air chamber (12), over the boundary surface (a), and the excess spray is absorbed by means of the substrate aerosol evacuating device (4). The micro-organisms growing in an emerged manner are located in the nutrient solution chamber (11) along the boundary surface (a).