CLINICAL ASSAY FOR NUCLEIC ACIDS AMPLIFIED IN SOLID MATRICES TO PRODUCE COLONIES OF THE PROGENY OF INDIVIDUAL TARGET MOLECULES

摘要

At least two nucleic acid targets of quantitative multiplex detection, the method that wherein rare and another this fluid appears in more high level, including addition reagent for expanding target, by polymerase chain reaction (PCR) to thin polyacrylamide gel matrix, that has been washed and has dried, distribution sample contains the surface that target passes through gel, but the molecular target for being not enough to separate rare target molecule is abundant, it recycles the gel and expands target protocol by PCR, count the rare target of number of individual bacterium colony.