BIOSYNTHETIC GENES OF BLAKESLEA TRISPORA BETA-CAROTENE THAT CODE FOR LYCOPENE CYCLASE/PHYTOENE SYNTHASE (CARRP) AND PHYTOENE DEHYDROGENASE (CARB)

摘要

Biosynthetic genes of β-carotene from Blakeslea trispora that code for lycopene cyclase / phytoene synthase (carRP) and phytoene dehydrogenase (carB). The carRP gene, characterized by the DNA sequence SEQ ID NO:1, codes for the amino acid sequence SEQ ID NO:3, which has the lycopene cyclase / phytoene synthase enzymes. For its part, the carB gene, characterized by the DNA sequence SEQ ID NO:2, codes for the amino acid sequence SEQ ID NO:4, which possesses phytoene dehydrogenase enzyme activity. In addition, (i) procedures for constructing plasmids for the expression of additional copies of the aforesaid genes and (ii) methods for the expression of heterologous genes under the control of the promoters of said genes, are described.