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/ A Gene-Defective Cyanobactria Method for Increasing Resistancy against Salts and Increasing Productivity of PHB using the Same Bacteria

机译:/一种使用相同细菌提高抗盐性和提高PHB生产率的基因缺陷型蓝细菌方法

摘要

PURPOSE: A gene-defective Cyanobacteria, and methods for increasing salt resistance of plants and productivity of PHB(poly-beta-hydroxybutyrate) using the same are provided. The productivity of plants is improved by minimizing damage by salts. CONSTITUTION: Cta1 gene-defective Cyanobacteria, Synechocystis sp. PCC6083 Cta1 (KCTC 10247BP) is provided, wherein Synechocystis sp. PCC6083 Cta1 (KCTC 10247BP) is obtained by isolating Cta1 gene, damaging the isolated Cta1 gene, inserting the damaged Cta1 gene into a vector to construct a plasmid pGEM Z, and transforming Synechocystis sp. PCC6083 with pGEM Z using humongous recombination. A method for increasing productivity of PHB(poly-beta-hydroxybutyrate) comprises culturing Synechocystis sp. PCC6083 Cta1 (KCTC 10247BP) in a medium.
机译:目的:提供一种基因缺陷型蓝细菌,以及使用其提高植物的耐盐性和PHB(聚-β-羟基丁酸酯)生产率的方法。通过使盐的损害最小化来提高植物的生产率。组成:Cta1基因缺陷型蓝细菌,集胞藻。提供了PCC6083 Cta1(KCTC 10247BP),其中集胞藻属。 PCC6083 Cta1(KCTC 10247BP)是通过分离Cta1基因,破坏分离的Cta1基因,将受损的Cta1基因插入载体中以构建质粒pGEM Z并转化Synechocystis sp。而获得的。使用巨大重组,将PCC6083与pGEM Z结合使用。一种提高PHB(聚-β-羟基丁酸酯)生产率的方法,包括培养集胞藻。介质中的PCC6083 Cta1(KCTC 10247BP)。

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