1 1 Fluorescence-Labeled Peptide for ILK1 Protein Kinase Assay and Non-Radioactive ILK1 Protein Kinase Assay Using the Same

摘要

PURPOSE: A fluorescence-labeled peptide for protein kinase ILK1 assay and a non-radioactive protein kinase ILK1 assay using the same are provided, thereby easily, rapidly, accurately and stably determining the activity of purified or unpurified protein kinase ILK1 without using radioactive isotope. CONSTITUTION: A peptide for protein kinase ILK1 assay is characterized by having the amino acid sequence of SEQ ID NO: 13, wherein the peptide is selected from the amino acid sequences of SEQ ID NO: 1 to SEQ ID NO: 8; and the peptide is labeled with fluorescence. A method for determining activity of non-radioactive protein kinase ILK1 comprises the steps of: labeling a peptide for protein kinase ILK1 assay with fluorescence; reacting the fluorescence-labeled peptide with protein kinase ILK1; subjecting the reacted fluorescence-labeled peptide to electrophoresis to separate phosphorylated and unphosphorylated peptides; and determining the quantity of phosphorylated peptide.