/ / 5'- APLYSIA CCAAT ENHANCER-BINDING PROTEIN ISOLATED FROM APLYSIA KURODAI AND 5'-FLANKING REGION

摘要

PURPOSE: An Aplysia CCAAT enhancer-binding protein(ApC/EBP) isolated from Aplysia kurodai and a 5'-flanking region are provided, thereby increasing expression of ApC/EBP related to long-term memory and consequently improving the long-term memory. CONSTITUTION: A polynucleotide encoding Aplysia CCAAT enhancer-binding protein(ApC/EBP) comprises the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 8, wherein the polynucleotide has the nucleotide sequence set forth in SEQ ID NO: 7. A C/EBP(CCAAT-enhancer binding protein long form) polynucleotide comprises the amino acid sequence set forth in SEQ ID NO: 29. A C/EBP(CCAAT-enhancer binding protein short form) polynucleotide comprises the amino acid sequence set forth in SEQ ID NO: 30. A C/EBP 5-flanking region polynucleotide comprises the amino acid sequence set forth in SEQ ID NO: 28. A polynucleotide encoding Aplysia ribosome protein 4(ApS4) comprises the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 10, wherein the polynucleotide has the nucleotide sequence set forth in SEQ ID NO: 9. A vector containing the C/EBP 5-flanking region and a foreign gene is provided, wherein the vector expresses the foreign gene when 5-HT is present; the C/EBP 5-flanking region is selected from the nucleotide sequences set forth in SEQ ID NO: 24, SEQ ID NO: 25 and SEQ ID NO: 28; and the foreign gene is selected from GFP gene, luciferase gene and LacZ gene.