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CANCER APOPTOTIC INDICATION VECTOR AND CANCER APOPTOTIC EXPRESSION PROMOTER
CANCER APOPTOTIC INDICATION VECTOR AND CANCER APOPTOTIC EXPRESSION PROMOTER
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机译:癌症细胞凋亡指示载体和癌症细胞凋亡表达促进剂
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摘要
PURPOSE: A cancer apoptotic indication vector and a cancer apoptotic expression promoter are provided, thereby screening compounds selectively inducing the apoptosis of various cancer cells. CONSTITUTION: The cancer apoptotic indication vector comprises p53 DNA binding motif comprising the common DNA binding sequence of p53 having the nucleotide sequence set forth in SEQ ID NO: 1; a minimum promoter of thymine kinase gene of Herpes simplex virus at nucleotide position -81 to +52; and a detection marker bound to the promoter selected from luciferase, beta-galactosidase and green fluorescent protein, wherein the vector may further comprises a selection marker selected from zeosine, hygromycine and neomycin. The cancer apoptotic expression promoter hBubR1/hMad3 has the nucleotide sequence set forth in SEQ ID NO: 2.
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机译:目的:提供癌症凋亡指示载体和癌症凋亡表达启动子,从而筛选选择性诱导各种癌细胞凋亡的化合物。组成:癌症凋亡指示载体包含p53 DNA结合基序,该基序包含具有SEQ ID NO:1所示核苷酸序列的p53的常见DNA结合序列。单纯疱疹病毒胸腺嘧啶激酶基因在-81至+52位核苷酸的最小启动子;以及与选自荧光素酶,β-半乳糖苷酶和绿色荧光蛋白的启动子结合的检测标记,其中所述载体可以进一步包含选自以下的选择标记:玉米素,潮霉素和新霉素。癌细胞凋亡表达启动子hBubR1 / hMad3具有SEQ ID NO:2所示的核苷酸序列。
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