Detection kit for the classical swine fever antibody using immunochromatography method

摘要

PURPOSE: A detection kit for swine cholera virus antibody using immunochromatography method is provided, thereby rapidly detecting the swine cholera virus antibody, and enabling the in-situ detection of the swine cholera virus antibody, so that the swine cholera virus antibody can be rapidly detected in farmhouses. CONSTITUTION: The detection kit for swine cholera virus antibody using immunochromatography method is prepared by binding a protein antigen(1), preferably E2 protein of swine cholera virus to strip type nitrocellulose membrane(5); and binding anti-swine IgG antibody(2) to the opposite side of the nitrocellulose membrane(5). The method for detecting the swine cholera virus antibody comprises the steps of: contacting a swine blood sample with the nitrocellulose membrane(5) by spotting the sample on the sample pad through a sample loading hole(4); transferring the swine blood sample to the swine cholera virus antigen(1) via the nitrocellulose membrane(5), and transferring the anti-swine IgG gold conjugate adsorbed in a conjugate pad to the absorbing pad(3); and measuring the occurrence of red purple by the antigen-antibody reaction followed by the gold particle reaction with the antigen-antibody conjugate in the antigen fixed region(1).