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TRANSGENIC MUD LOACHOVER(MISGURNUS MIZOLEPIS) CONTAINING APOLIPOPROTEIN AI GENE AND METHOD FOR PRODUCING THE SAME TO MASS-PRODUCE HIGH DENSITY OF LIPOPROTEIN(HDL)
TRANSGENIC MUD LOACHOVER(MISGURNUS MIZOLEPIS) CONTAINING APOLIPOPROTEIN AI GENE AND METHOD FOR PRODUCING THE SAME TO MASS-PRODUCE HIGH DENSITY OF LIPOPROTEIN(HDL)
PURPOSE: Transgenic mud loachover(Misgurnus mizolepis) containing apolipoprotein AI gene and a method for producing the same are provided, which transgenic mud loachover(Misgurnus mizolepis) over-express the apolipoprotein AI gene, thereby mass-producing high density of lipoprotein(HDL) which regulates cholesterol metabolism in vivo. CONSTITUTION: The method for producing the transgenic mud loachover(Misgurnus mizolepis) overproducing high density of lipoprotein-cholesterol(HDL-CHO) comprises the steps of: constructing an expression vector containing cDNA of mud loachover(Misgurnus mizolepis) apolipoprotein AI having the nucleotide sequence of SEQ ID NO:1; micro-injecting the cDNA of mud loachover(Misgurnus mizolepis) apolipoprotein AI containing expression vector into Misgurnus mizolepis; and hatching the transgenic mud loachover(Misgurnus mizolepis), wherein the expression vector is pFV4mApoA I; and the mud loachover(Misgurnus mizolepis) apolipoprotein AI has the amino acid sequence of SEQ ID NO:2.
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机译：目的：提供一种含载脂蛋白AI基因的转基因泥lo（Misgurnus mizolepis）及其生产方法，该基因可以过量表达载脂蛋白AI基因，从而大量生产高密度脂蛋白（HDL）在体内调节胆固醇的代谢。组成：生产高密度脂蛋白胆固醇（HDL-CHO）的高密度转基因泥lo（Misgurnus mizolepis）的方法包括以下步骤：构建包含具有核苷酸序列的泥lo（Misgurnus mizolepis）载脂蛋白AI cDNA的表达载体SEQ ID NO：1的;将含有表达载体的泥lo（Misgurnus mizolepis）载脂蛋白AI的cDNA微注射到Misgurnus mizolepis中;孵化转基因泥lo（Misgurnus mizolepis），其中表达载体为pFV4mApoAI。泥lo（Misgurnus mizolepis）载脂蛋白AI的氨基酸序列为SEQ ID NO：2。
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