UBIQUITIN-SPECIFIC PROTEASES FROM CHICK MUSCLE WHICH IS USEFUL FOR DEVELOPMENT OF ANTICANCER DRUGS RELATED TO P53 PROTEIN AND GENE THEREOF

摘要

PURPOSE: Ubiquitin-specific proteases from chick muscle and gene thereof are provided. The enzymes remove ubiquitin bound to p53 protein to stabilize p53 protein in a cell, so that they can be useful for development of anticancer drugs related to p53 protein. CONSTITUTION: The ubiquitin-specific proteases from chick muscle are UBP75a, UBP75b and UBP135, wherein the ubiquitin-specific protease digests protein or peptide bound to ubiquitin by alpha-peptide bond; the ubiquitin-specific protease digests protein or peptide bound to ubiquitin by epsilon-peptide bond; the ubiquitin-specific protease comprises the total or portion of the amino acid sequence of SEQ ID NO:4, SEQ ID NO:5 or SEQ ID NO:6; and UBP75a, UBP75b and UBP135 have molecular weight of 75 kDa, 75 kDa and 135 kDa, respectively. The ubiquitin-specific protease genes are ubp75a, ubp75b and ubp135, wherein the ubiquitin-specific protease genes are isolated from cDNA of chicken; genes ubp75a, ubp75b and ubp135 have the total or portion of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, respectively. The expression vectors pBS-UBP75a, pBS-UBP75b and pBS-UBP135 contains the genes ubp75a, ubp75b and ubp135, respectively.