A Molecular Marker for Colletotrichum gloeosporioides in Sweet persimmon

摘要

PURPOSE: A molecular marker, particularly, DNA marker containing a pair primer, ITS-4 and CO-1, is provided which is used for the diagnosis of anthracnose disease in a sweet persimmon. It detects the infection of anthrax bacteria, Colletotrichum spp, at a minimum concentration of 10pg, thereby diagnosing anthracnose disease at its early stage and consequently preventing over-use of pesticides for anthracnose control. CONSTITUTION: The anthrax bacterium, Colletotrichum gloeosporioides, causes anthracnose disease in a sweet persimmon. The DNA marker for the diagnosis of anthracnose disease in a sweet persimmon is produced by amplification using a pair primer ITS-4 having nucleotide sequence of SEQ ID NO:1 (tcctccgctt attgatatgc), and CO-1 having nucleotide sequence of SEQ ID NO:2 (ataaccttt gtgaacatac).