Multitest-Microslide of living cell cultures and culture slide assembly and their preparation

摘要

PURPOSE: A multitest-microslide of living cell cultures, a culture slide assembly and a preparation method thereof are provided, thereby confirming the inserted gene expression and expression position in transformed cells using a microscope. CONSTITUTION: A multitest-microslide comprises 2 to 100 wells where living cells are cultured. A kit for culturing living cells comprises the multitest-microslide comprising 2 to 100 wells; a culturing vessel comprising a fixing means for fixing the multi-test microslide and a humidity-maintaining means for maintaining humidity in the air; and a cover. A method for preparing the kit for culturing living cells comprises the steps of: coating slideglass with hydrophobic film wherein 2 to 100 wells are indicated, sterilizing the slideglass under high pressure, adding gelatin solution into each well, and drying the slideglass to prepare the multitest-microslide; installing the fixing means in the culturing vessel and installing the humidity-maintaining means along the bottom of the culturing vessel; and preparing the cover.