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Method for extracting tooth protein from extracted tooth

机译:从提取的牙齿中提取牙齿蛋白的方法

摘要

PURPOSE: A method for extracting tooth protein from an extracted tooth and the use thereof are provided, thereby easily and rapidly extracting the tooth protein. CONSTITUTION: A method for extracting tooth protein from an extracted tooth comprises the steps of: washing the extracted tooth with enamel with distilled water; pulverizing the tooth, washing the pulverized tooth, removing fat from the pulverized tooth using organic solvent, and removing lime from the pulverized tooth; and removing fat from the lime-removed tooth, washing the tooth and extracting tooth protein using 4M guanidine-HCl, wherein the extracted tooth protein is optionally subjected to electrophoresis and is optionally adsorbed to the PVDF membrane. The extracted tooth protein is adsorbed to chitosan.
机译:目的:提供一种从提取的牙齿中提取牙齿蛋白的方法及其用途,从而容易且快速地提取牙齿蛋白。组成:从拔牙器中提取牙齿蛋白质的方法包括以下步骤:用搪瓷和蒸馏水洗涤拔牙器;磨碎牙齿,洗净磨碎的牙齿,使用有机溶剂从磨碎的牙齿上去除油脂,并从磨碎的牙齿上去除石灰;从去除石灰的牙齿中去除脂肪,洗涤牙齿并使用4M盐酸胍提取牙齿蛋白,其中将提取的牙齿蛋白任选地进行电泳并且任选地吸附到PVDF膜上。提取的牙齿蛋白被吸附到壳聚糖上。

著录项

  • 公开/公告号KR100450146B1

    专利类型

  • 公开/公告日2004-09-30

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20020008789

  • 发明设计人 정필훈;

    申请日2002-02-19

  • 分类号C07K1/14;

  • 国家 KR

  • 入库时间 2022-08-21 22:46:35

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