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Method for extracting tooth protein from extracted tooth
Method for extracting tooth protein from extracted tooth
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机译:从提取的牙齿中提取牙齿蛋白的方法
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摘要
PURPOSE: A method for extracting tooth protein from an extracted tooth and the use thereof are provided, thereby easily and rapidly extracting the tooth protein. CONSTITUTION: A method for extracting tooth protein from an extracted tooth comprises the steps of: washing the extracted tooth with enamel with distilled water; pulverizing the tooth, washing the pulverized tooth, removing fat from the pulverized tooth using organic solvent, and removing lime from the pulverized tooth; and removing fat from the lime-removed tooth, washing the tooth and extracting tooth protein using 4M guanidine-HCl, wherein the extracted tooth protein is optionally subjected to electrophoresis and is optionally adsorbed to the PVDF membrane. The extracted tooth protein is adsorbed to chitosan.
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