Immunoassay method for determining the soluble form of HLA-DR antigens in human serum

摘要

FIELD: medicine, immunology.;SUBSTANCE: immobilization of specific antibodies is carried on solid carrier followed by the successive incubation with analyzed serum and peroxidase conjugate. Polyclonal antibodies raised against surface antigens of human mononuclear cells are used as the first antibodies, and HLA-DR-specific monoclonal antibodies ИКО-1 conjugated with horse radish peroxidase are used as the second antibodies.;EFFECT: improved assay method.;3 cl, 1 tbl, 1 dwg, 1 ex