METHOD FOR PREPARING BACCATIN AND/OR DERIVATIVES OF BACCATIN, 10-HYDROXYTAXO-O-ACETYLTRANSFERASE FOR ITS REALIZATION, METHOD FOR PREPARING THIS ACETYLTRANSFERASE AND METHOD FOR PREPARING TAXOL AND/OR DERIVATIVES OF TAXOL

摘要

FIELD: biotechnology, biochemistry. SUBSTANCE: method for preparing baccatin and/or derivatives of baccatin involves conversion of 10-desacetylbaccatin or derivative of 10-desacetylbaccatin in the presence of 10- hydroxytaxo-O-acetyltransferase and donor of acetyl group. Acetyltransferase with molecular mass 70-72 kDa is used determined by electrophoresis on polyacrylamide gel in the presence of sodium dodecyl sulfate that is obtained from cellular cultures of Taxus chinensis. Invention provides high regiospecificity with respect to acetylation at position 10 that is above 99%. EFFECT: improved preparing method. 13 cl, 5 tbl