DNA SEQUENCE ENCODING EHRLICHIA CANIS PROTEIN WITH MOLECULAR MASS 30 KDA, VECTOR, RECOMBINANT PROTEIN AND METHOD FOR IT PREPARING

摘要

FIELD: molecular biology, genetic engineering.;SUBSTANCE: invention proposes DNA sequence encoding Ehrlichia canis protein with molecular mass 30 kDa. Also, invention proposes vector for preparing protein comprising DNA sequence, recombinant protein for preparing antibodies taken among the group consisting of SEQ ID NO:4 and 6, and a method for it preparing. Recombinant protein from Ehrlichia canis with molecular mass 30 kDa shows immune reactivity to serum raised against Ehrlichia canis. Proposed group of inventions can be used for the development of vaccine against canine ehrlichiosis.;EFFECT: improved preparing method, valuable properties of protein.;12 cl, 10 dwg, 18 ex