Retroviral vectors with an externally sequence insertion between a retroviral primer binding site and a viral splice donor

摘要

A retroviral vector having the general structure5 ''– [Ltr] – [pbs] – [sd] – [ψ] – [sa] – [pp] – [ltr] - 3'' ,in the case of the[Ltr] the terminal terminal repeat sequence (long terminal repeat, ltr) of the retroviral vector is,[Pbs] the retroviral primer binding site is,[Sd] the retroviral splice donor signal is,[Ψ] the retroviral packaging signal is,[Sa] the retroviral splice acceptor signal is,and[Pp] is a poly purine tract (pp - tract) is,characterized in thatthe vector between the retroviral primer binding site (pbs) and the retroviral splice donor (sd) a foreign sequence [fs1]; the one enhancer - promoter - sequence, enhancers - sequence, cdna - sequence, a sequence for matrix device - adhesion between the regions (mar), and / or a sequence for rna - they ethyl 1-cyclopropyl-7-fluoro-8-trifluoromethyl-1,4-dihydro-4-oxo-3-quinolinecarboxylate control signals is.