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Improving pathogen resistance of plants, e.g. of cereals against fungi, by reducing activity of a RacB protein, also new proteins and nucleic acids encoding them

机译:改善植物对病原体的抵抗力谷物通过降低RacB蛋白,新蛋白和编码它们的核酸的活性来对抗真菌

摘要

Inducing or increasing (M1) resistance of plants to at least one pathogen comprising: (a) reducing the amount, activity or function of a RacB protein (I) in a plant, or its tissue, organ, part or cell; and (b) selecting plants for increased resistance, relative to the parent plant, is new. Independent claims are also included for: (1) barley RacB protein (Ia) having a 197 amino acid (aa) sequence (2), reproduced; (2) polypeptides (Ib) of 212, 197 or 213 aa (reproduced) that are functional equivalents of (Ia); (3) dominant-negative variant (Ic) of (Ia); (4) nucleic acid sequences (II) that encode (Ia), (Ib) or (Ic); (5) double-stranded RNA (III) that reduces expression of RacB; (6) transgenic expression cassette (EC) containing a promoter linked to (II) or a sequence that encodes (III); (7) transgenic vector containing EC; and (8) transgenic organism containing (II), sequence encoding (III), EC or the vector. ACTIVITY : Plant Antibacterial; Plant Antifungal; Insecticide; Plant Antiviral; Nematocide. No suitable data given. MECHANISM OF ACTION : Modulation of RacB which is a key element in successful penetration of pathogens into plant cells.
机译:诱导或增加植物对至少一种病原体的抗性(M1),包括:(a)降低植物或其组织,器官,部分或细胞中RacB蛋白(I)的量,活性或功能; (b)选择相对于亲本植物具有更高抗性的植物是新的。还包括以下方面的独立权利要求:(1)复制的具有197个氨基酸(aa)序列的大麦RacB蛋白(Ia)(2); (2)(Ia)的功能等同物的212、197或213aa的多肽(Ib)(复制的); (3)(Ia)的显性-阴性变体(Ic); (4)编码(Ia),(Ib)或(Ic)的核酸序列(II); (5)降低RacB表达的双链RNA(III); (6)转基因表达盒(EC),其含有与(II)连接的启动子或编码(III)的序列。 (7)含有EC的转基因载体; (8)转基因生物,其含有(II),编码(III),EC或载体的序列。活性:植物抗菌;植物抗真菌剂杀虫剂植物抗病毒杀线虫剂。没有给出合适的数据。作用机理:RacB的调节是成功地将病原体渗透到植物细胞中的关键因素。

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