New nucleic acid encoding inactive L-serine dehydratase, useful for improving bacterial synthesis of L-serine, is a deleted or mutated form of the wild-type sequence

摘要

Nucleic acid (I), optionally recombinant and replicable in Corynebacterium, has the sequence for L-serine dehydratase (II): (a) partly or completely deleted or mutated; or (b) expressed at a lower level, relative to the natural sequence, or not at all. Independent claims are also included for: (1) gene structure (GS) comprising (I) and linked regulatory elements; (2) vector containing (I) or GS, also sequences for selection, replication in a host cell or integration into the host cell genome; (3) (II) with reduced activity, encoded by (I); (4) microorganism that contains (I); (5) probe for identification and/or isolation of genes that encode proteins involved in biosynthesis of L-serine, derived from (I) and labeled to allow detection; and (6) microbial production of L-serine by growing microorganisms of (4).