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liver parenchymale cells with klonaler growth capacity, method of manufacture and system of primary hepatocyten
liver parenchymale cells with klonaler growth capacity, method of manufacture and system of primary hepatocyten
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机译:具有克隆生长能力的肝实质男性细胞,原肝细胞的制造方法和系统
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摘要
PURPOSE: To obtain a subculture medium which can continue the proliferation of primarily cultured hepatic cells for many generations, thus is useful for elucidation of characteristics and action mechanisms of living tissues and their cells by adding a nicotinamide and an ascorbic acid to the medium for the primary hepatic cell culture system. ;CONSTITUTION: The medium for the primary hepatic cell culture such as DMEM medium is combined with a nicotinamide and an ascorbic acid as essential components, optionally further FCS and EGF. The nicotinamide promotes the proliferation of committed progenitor cells, while L-ascorbic acid 2-phosphate accelerates the proliferation of hepatic cells to maintain the survival of subcultured hepatic cells.;COPYRIGHT: (C)1995,JPO
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