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renilla luciferase and green fluorescent protein fusion genes
renilla luciferase and green fluorescent protein fusion genes
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机译:海肾荧光素酶和绿色荧光蛋白融合基因
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摘要
A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the 'humanized' Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the 'Renilla-GFP fusion protein', which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.
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