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Brief description of the detection of fungi producers ochratoxin a or citrinine

机译:真菌生产者曲霉毒素a或柑桔碱的检测的简要说明

摘要

Isolated proteins (A) that contain either of two specified sequences. Isolated proteins (A) that contain either of the sequences Arg-Glu-Ala-Leu-Ala-Met-Asp-Pro-Gln-Gln-X1-Leu-X2-Glu-X3-X4-Tyr-Glu (41) or Thr-Xa-Phe-Val-Glu-Xb-His-Gly-Thr-Gly-Thr-Gln-Xc-Gly-Asp (43) X1 = Leu, Val, Met or Ile; X2 = Leu or Met; X3 = Ala or Thr; X4 = Val or Ala; Xa = Asp or Ala; Xb = Cys or Ala; and Xc = Phe or Leu. Independent claims are also included for: (1) a nucleic acid (B) that encodes (A); (2) primers for amplifying (B) containing 18-25 consecutive bases from (B); (3) a method for detecting fungi that produce ochratoxin A or citrinine by detecting (B); and (4) a DNA chip having at least one (B) on its surface.
机译:包含两个指定序列之一的分离蛋白(A)。分离的蛋白质(A),其包含以下序列之一:Arg-Glu-Ala-Leu-Ala-Met-Asp-Pro-Gln-Gln-X1-Leu-X2-Glu-X3-X4-Tyr-Glu(41)或Thr-Xa-Phe-Val-Glu-Xb-His-Gly-Thr-Gly-Thr-Gln-Xc-Gly-Asp(43)X1 = Leu,Val,Met或Ile; X2 = Leu或Met; X3 =丙氨酸或苏氨酸; X4 = Val或Ala; Xa =天冬氨酸或丙氨酸; Xb =半胱氨酸或丙氨酸; Xc = Phe或Leu。还包括以下方面的独立权利要求:(1)编码(A)的核酸(B); (2)用于扩增(B)的引物,其含有来自(B)的18-25个连续的碱基; (3)一种通过检测(B)来检测产生曲霉毒素A或青霉素的真菌的方法。 (4)在表面具有至少一个(B)的DNA芯片。

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