New recombinant triacylglycerol lipase from Arabidopsis thaliana, useful e.g. for treating lipase deficiency, also nucleic acid for preparation of transgenic plants

摘要

Recombinant triacylglycerol (TAG) lipase (I) of Arabidopsis thaliana, and its derivatives and/or variants, are new. Recombinant triacylglycerol (TAG) lipase (I) of Arabidopsis thaliana, and its derivatives and/or variants, are new. (I) has a sequence (2) of 393 amino acids (aa), where the first 21 aa represent a signal peptide, and the mature protein has a 372 aa sequence (4). The variants/fragments may have one or more aa substitutions provided that they retain: (i) at least 70% identity or 80% similarity with (I); and (ii) have the same enzymatic activity as (2), especially ability to hydrolyze oils such as long-chain TAG, specifically triolein. Independent claims are also included for: (1) nucleic acid sequence (II) that encodes (I) or its fragments or variants; (2) recombinant nucleic acid (IIa) that comprises (II) and elements required for transcription, particularly promoter and terminator; (3) vectors e.g. plasmids or baculovirus, that contains (IIa); (4) host cells transformed by the vector of (3); (5) preparation of (I), or its fragments or variants, by culturing cells of (4); (6) antisense nucleic acids (IIb) that are complementary to (II); (7) vector e.g. plasmid or Agrobacterium tumefaciens that contains (IIb); (8) transgenic plant cells, plant parts or plants transformed with (II), (IIa) or (IIb); (9) transgenic seeds transformed with (II), (IIa) or (IIb), produced by crossing plants of (8), and the plants produced by growing them; (10) methods for producing transgenic plants of (8); (11) nutritional supplement or pharmaceutical composition that contains (I), or its fragments or variants; and (12) mono- or poly-clonal antibodies (Ab) directed against (I), or its derivatives and fragments.