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Isolating endotoxins from bacteria or culture supernatant, useful e.g. in vaccines, by suspension in solvent mixture, separating suspended particles and removing solvent from the supernatant
Isolating endotoxins from bacteria or culture supernatant, useful e.g. in vaccines, by suspension in solvent mixture, separating suspended particles and removing solvent from the supernatant
Isolating endotoxins (I) from bacteria or culture supernatants comprising suspending the starting material in a mixture of solvents; separating suspended particles from the solution and removing solvent from the supernatant or filtrate, is new. Isolating endotoxins (I) from bacteria or culture supernatants comprises suspending the starting material in a mixture of solvents; separating suspended particles from the solution and removing solvent from the supernatant or filtrate. (A) comprises 30-70% 3-6C linear or branched aliphatic acid (II) and 70-30% basic aqueous solution of 0-12C aliphatic amine (III), and the mixture of starting materials and (A) is stirred to keep particles in suspension. Separation is by filtration, centrifugation and recovery of the supernatant, or by centrifugation and then filtration of the supernatant. An Independent claim is also included for a method for preparing a composition containing at least one (I), or its derivatives, for administration to humans or animals, that includes the new method for isolating (I).
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