New hyperactive mutant of the mariner transposase, useful for targeted insertion of DNA, particularly for gene therapy, has at least one phosphorylatable amino residue replaced by non-phosphorylatable residue

摘要

A hyperactive transposase mutant (A) from a mariner mobile genetic element (EGM) is new. It has at least one mutation of a phosphorylatable residue (pR), in at least one phosphorylation site, that renders the site non-phosphorylatable. A hyperactive transposase mutant (A) from a mariner mobile genetic element (EGM) is new. It has at least one mutation of a phosphorylatable residue (pR), in at least one phosphorylation site, that renders the site non-phosphorylatable. pR is any of Thr 24, 42, 88, 135, 154, 181, 216 or 255; Ser 28, 104, 147, 170 or 305; or Tyr 171, in a 345 amino acid (aa) sequence (S2), defined in the specification, or at corresponding sites in other mariner EGM, aligned with (S2). Independent claims are also included for the following: (1) recombinant nucleic acid (I) that encodes (A); (2) recombinant vector containing at least one (I); (3) recombinant host cell that harbors at least one vector of (2); and (4) method for producing (A).