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APPARATUS FOR EXTRACTING DNA AND METHOD FOR EXTRACTING DNA

机译:提取DNA的装置和提取DNA的方法

摘要

PROBLEM TO BE SOLVED: To simply carry out processes from cell culture to DNA extraction.;SOLUTION: A cell is cultured in a cell culture chamber 5 and a metal ion capable of producing a chelate compound between the metal ion and a protein and a lysozyme are fed from a liquid inlet 3 to the cell and a cell membrane of the cell is broken by heating and the chelate compound is produced from the protein and its metal ion. Then, an eluate is fed from the liquid inlet 3 and a protein 16 producing the chelate compound is blocked by a filter 10 and a DNA 14 is extracted by permeating the DNA 14 through the filter 10.;COPYRIGHT: (C)2005,JPO&NCIPI
机译:解决的问题:简单地进行从细胞培养到DNA提取的过程。;解决方案:将细胞在细胞培养室5中培养,金属离子能够在金属离子与蛋白质和溶菌酶之间产生螯合化合物液体从液体入口3被送入细胞,并通过加热破坏细胞的细胞膜,并由蛋白质及其金属离子产生螯合化合物。然后,从液体入口3进料洗脱液,并通过过滤器10阻断产生螯合化合物的蛋白质16,并且通过使DNA 14穿过过滤器10而提取DNA14。版权所有:(C)2005,JPO&NCIPI

著录项

  • 公开/公告号JP2005261389A

    专利类型

  • 公开/公告日2005-09-29

    原文格式PDF

  • 申请/专利权人 SHIMADZU CORP;

    申请/专利号JP20040082772

  • 发明设计人 OIKAWA YUKIO;

    申请日2004-03-22

  • 分类号C12N15/09;

  • 国家 JP

  • 入库时间 2022-08-21 22:35:02

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